Kudzidza kwedu kunoratidza kuti Ganoderma lucidum/reishi/lingzhi inoratidza antitumor zvivakwa pane osteosarcoma masero in vitro.Yakawanikwa kuti Ganoderma lucidum inhibits cancer cell cell kukura uye kutama nekudzvinyirira Wnt/β-catenin signing.Iyo inodzvanya kenza yemapapu kuburikidza nekuvhiringidzwa kwekunamira kwekunamatira uye induction yeMDM2-mediated Slug degradation.Ganoderma lucidum inhibits cancer yemazamu kuburikidza nekudzikisira iyo PI3K/AKT/mTOR nzira, Ganoderma lucidum inotora antitumor basa muacute leukemia masero nekuvharira iyo MAPK nzira.
CCK-8 uye colony formation assays, yekuongorora mhedzisiro yeGanoderma lucidum pane osteosarcoma cell line viability uye kupararira, yakaratidza kuti Ganoderma lucidum inodzvinyirira kupararira kweMG63 uye U2-OS masero mune imwe nguva- uye kufunga-inotsamira nzira, uye inoderedza iyo kugona kwemaseru kuita colonize.
Ganoderma lucidum inosimudzira kutaura kweproapoptotic majini, uye kuyerera kwecytometry kuongororwa kwakaratidza kuti apoptosis yeMG63 uye U2-OS masero inowedzerwa mushure mekurapwa neGanoderma lucidum.Kutama kwesero ndiyo hwaro hweakasiyana maitiro ebhayoloji, anosanganisira angiogenesis, kuporeswa kwemaronda, kuzvimba, uye metastasis yegomarara.Ganoderma lucidum inoderedza kutama uye kupinda kweese mitsara yesero uye inodzivirira kuwanda, kutama, uye kupinda, uye induces apoptosis ye osteosarcoma masero.
Aberrant Wnt / β-catenin siginecha yakanyatsoenderana nekuumbwa, metastasis, uye apoptosis yemhando dzakawanda dzekenza, nekukwidziridzwa kweWnt / β-catenin kusaina kunoonekwa mune osteosarcoma.
Muchidzidzo ichi, dual-luciferase reporter assays yakaratidza kuti Ganoderma lucidum kurapwa inovhara CHIR-99021-activated Wnt/β-catenin signing.Izvi zvinowedzerwa nekuratidzwa kwedu kuti kunyorwa kweWnt target genes, zvakadai seLRP5, β-catenin, cyclin D1, uye MMP-9, inodziviswa apo masero e osteosarcoma anobatwa neGanoderma lucidum.
Zvidzidzo zvakapfuura zvakaratidza mumakiriniki samples kuti LRP5 inokwidziridzwa muosteosarcoma inoenderana neyakajairwa tishu, uye kutaura kweLRP5 inowirirana nechirwere chemetastatic uye kusapona kwechirwere-chisina chirwere, zvichiita kuti LRP5 ive inogona kurapa chinangwa cheosteosarcoma.
β-catenin pachayo ndiyo inonyanya kukosha muWnt / β-catenin nzira yekucherechedza, uye kutaura kwe β-catenin mu osteosarcoma kunowedzera zvakanyanya.Kana β-catenin ichipinda mukati meiyo nucleus kubva ku cytoplasm, inomutsa kutaura kweiyo yakadzika yakananga majeni, ayo anosanganisira cyclin D1, C-Myc, uye MMPs.
Myc ndeimwe yeakanyanya proto-oncogenes uye inoita basa rakakosha mukugadzirisa activation, transcription, uye inhibition yegene expression.Zvakashumwa kuti kudzvinyirirwa kweC-Myc oncogene kunoita kuti kuchembera uye apoptosis yemhando dzinoverengeka dze tumor cell, kusanganisira. osteosarcoma.
Cyclin D1 yakakosha sero kutenderera G1 phase regulator uye inomhanyisa G1/S chikamu shanduko.Kuwedzeredza kutaura kwecyclin D1 kunogona kupfupisa kutenderera kwesero uye kukurudzira kukurumidza kuwanda kwesero mumhando dzakasiyana dzebundu.
MMP-2 uye MMP-9 ma stromelysins ane kugona kushatisa extracellular matrix zvikamu, chinhu chakakosha chebundu angiogenesis uye kupinda.
Izvi zvinoratidza kuti Wnt/β-catenin chinangwa chemajini chinoita basa guru mukufambira mberi kweosteosarcoma, uye kuti kuvhara idzi node dzechiratidzo kunogona kuva nemigumisiro inoshamisa yekurapa.
Zvadaro, takaona kutaura kwemRNA neprotein yeWnt / β-catenin-inoenderana-inotarirwa genes nePCR uye western blotting.Mumitsetse yese yesero, Ganoderma lucidum yakavharira kutaura kweaya mapuroteni nemajini.Migumisiro iyi inowedzera kuratidza kuti Ganoderma lucidum inhibits Wnt/β-catenin kusaina nekunongedza LRP5, β-catenin, C-Myc, cyclin D1, MMP-2, uye MMP-9.
E-cadherin inonzi transmembrane glycoprotein inoratidzwa zvakanyanya mumaseru epithelial uye inopindirana kunamatira pakati peepithelial masero uye stromal masero.Kudzimwa kana kurasikirwa kweE-cadherin kutaura kunotungamira mukurasikirwa kana kupera simba kwekunamatira pakati pemaseru ebundu, zvichiita kuti masero ebundu afambe zviri nyore, uyezve kuita kuti bundu ripinde, ripararire, uye riite metastasize.Muchidzidzo ichi, takaona kuti Ganoderma lucidum inogona kukwidza E-cadherin, nokudaro inopesana neWnt / β-catenin-mediated phenotype ye osteosarcoma masero.
Mukupedzisa, mhedzisiro yedu inoratidza kuti Ganoderma lucidum inovhara osteosarcoma Wnt/β-catenin kusaina uye pakupedzisira inotungamira mukudzikira kweosteosarcoma cell chiitiko.Izvi zvakawanikwa zvinoratidza kuti Ganoderma lucidum inogona kuve inobatsira uye inoshanda yekurapa mumiriri wekurapa osteosarcoma, izvo zvine hukama zvigadzirwa zvinosanganisira.ganoderma lucidum spore mafuta softgels/reishi spore oil softgels,ganoderma lucidum spore poda/reishi spore poda
Nguva yekutumira: Kubvumbi-18-2022